Liposomal clodronate therapy is a very useful drug formulation for treating autoimmune hemolytic anemia. This particular autoimmune disease destroys red blood cells. Usual therapies include the use of corticosteroids and splenectomy, but this one gives very fast and promising results by destroying macrophages.
Clodronate was successfully used for treating different osteolytic bone diseases. In various researches, it proved itself to be very useful in so called liposome mediated macrophage suicide technique. This method of depleting macrophages provides very good results in a very short time. This is especially important when such fast results are needed for successful treatment.
Clodronate itself cannot pass different cell membranes. When encapsulated within liposomes, they will surely be eagerly eaten by different macrophages. When the drug concentration reach the expected level within the macrophage cell, the result is the destruction of this cell. To be more precise, it is irreversibly damaged and dies by apoptosis.
The suspension itself is non-toxic. The released drug, once it reaches the circulation, is safely removed from the body by the renal system. The results are very fast, and although they aren't permanent, they can be very useful in different treatments.
Of course, this method is successful only if liposomal clodronate reaches the macrophages to destroy. Given the fact that liposomes cannot cross capillary walls, they can destroy the macrophage in the liver, lung, spleen, lymph nodes, joints and peritoneal cavity. If liposomes are adequately administered, they can also destroy macrophages in testis.
This method was designed for destroying macrophages in vivo. Although it can be used in in vitro research as well, the problem is that it cannot be removed from the medium so effectively this way. It means it could be accumulated in different cells, and affect your final results. In living organism, it is successfully removed once in circulation, because the kidneys take very good care about it.
The temperature is very important. The suspension should never be frozen, and it should never be heated above 30 degrees of Celsius. The ideal temperature for keeping it is 4 degrees of Celsius. In any case, the suspension should be used within a few days. It is important to shake it well before dividing it into smaller dosages, because it tends to precipitate. It is important to get an even distribution, to achieve the proper concentration.
The quantity may vary, but recommended intravenous injection should be less than 0,1 ml for every 10 grams of weight. This number can be higher in case of intraperitoneal injection of the drug. Clodronate is stored within the liposomes, and the concentration depends on its solubility.
Liposomal clodronate therapy will effectively destroy macrophages. The absence of macrophages may cause an increase in e. G., virus titers, bacteria or yeasts. Test animals should always be perfectly clean where injected, to avoid possible microbial contamination. You should always shake the syringe, to get a homogeneous suspension, especially if you use the same one on all your test animals, which is not recommended.
Clodronate was successfully used for treating different osteolytic bone diseases. In various researches, it proved itself to be very useful in so called liposome mediated macrophage suicide technique. This method of depleting macrophages provides very good results in a very short time. This is especially important when such fast results are needed for successful treatment.
Clodronate itself cannot pass different cell membranes. When encapsulated within liposomes, they will surely be eagerly eaten by different macrophages. When the drug concentration reach the expected level within the macrophage cell, the result is the destruction of this cell. To be more precise, it is irreversibly damaged and dies by apoptosis.
The suspension itself is non-toxic. The released drug, once it reaches the circulation, is safely removed from the body by the renal system. The results are very fast, and although they aren't permanent, they can be very useful in different treatments.
Of course, this method is successful only if liposomal clodronate reaches the macrophages to destroy. Given the fact that liposomes cannot cross capillary walls, they can destroy the macrophage in the liver, lung, spleen, lymph nodes, joints and peritoneal cavity. If liposomes are adequately administered, they can also destroy macrophages in testis.
This method was designed for destroying macrophages in vivo. Although it can be used in in vitro research as well, the problem is that it cannot be removed from the medium so effectively this way. It means it could be accumulated in different cells, and affect your final results. In living organism, it is successfully removed once in circulation, because the kidneys take very good care about it.
The temperature is very important. The suspension should never be frozen, and it should never be heated above 30 degrees of Celsius. The ideal temperature for keeping it is 4 degrees of Celsius. In any case, the suspension should be used within a few days. It is important to shake it well before dividing it into smaller dosages, because it tends to precipitate. It is important to get an even distribution, to achieve the proper concentration.
The quantity may vary, but recommended intravenous injection should be less than 0,1 ml for every 10 grams of weight. This number can be higher in case of intraperitoneal injection of the drug. Clodronate is stored within the liposomes, and the concentration depends on its solubility.
Liposomal clodronate therapy will effectively destroy macrophages. The absence of macrophages may cause an increase in e. G., virus titers, bacteria or yeasts. Test animals should always be perfectly clean where injected, to avoid possible microbial contamination. You should always shake the syringe, to get a homogeneous suspension, especially if you use the same one on all your test animals, which is not recommended.
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