ELISA is a medical test used in the enclosure of laboratories to figure out if a person has any certain disease or condition. It is an abbreviation from Enzyme Linked Immunosorbent Assay, where it tests the presence of certain protein molecules in a sample. Its main purpose is for testing the immunity of a patient.
HIV tests are one of the many examples of ELISA. It detects antibodies which are connected to the virus that are inside the blood of the patient. As for thyroid glands, Thyroid ELISA kits are used in most laboratories for further studying the present protein molecules. With this, other various kits are existent depending on what use they are.
In science and in certain industries, this test is used as one of the many tools of plant pathology and also with quality control check. Other examples this test is used for in diagnostic clinics are for food allergy and illegal drugs. The reading for this is determined by the vibrancy and intensity of the color changes in swabs whenever a sample is tested.
Two types of ELISA tests are widely used in laboratories. The first one is indirect which detects the antibodies in a given sample. An example for indirect is HIV testing, where it detects the antibodies in the sample which are against the virus. The second one is called capture or sandwich. It detects the antigens and then capture them between two antibodies. A good sample for this would be on pregnancy tests which detects the hCG or the human chorionic gonadotropin.
Blood is the usual collection, as well as urine samples. These samples are then placed in a test tube or test slide, and then sent to the laboratory for the testing and analysis. At the laboratory, it will be tested if the targeted antigen or antibody is present within the blood or urine.
The human blood samples inside the test tubes will be placed in a centrifuge to separate the different parts of it and for it to get a blood serum. A blood serum is a sample that has the clotting feature taken out of it. The high speed from the centrifuge separates the cells and plasma, making it easier to extract the serum.
Horseradish Peroxidase is an enzyme mostly used for detection where it separates Ortho Phenylenediamine Dihydrochloride and Tetramethylbenzidine. It results into a yellow color and for measuring the optical density, a plate reader would then be used. Light absorbance from OPD is 490 nanometers in wavelength, while TMB is at 450 nanometers.
If the patient has already been determined to have a disease or condition, the samples of their blood or urine will have certain antibodies which will be the ones to react to the test. These antibodies will stick to the antigens, which these antigens are bonding agents for the entire test. A solution is used to wash away everything in it except for the antigens, or also the antibodies that are sticking to the antigens.
Color changes, as mentioned before, are where you can achieve the results. Enzyme solutions are applied in the samples to get these results if they give out a positive result or not. There might be some instances where the sample from the patient has no known infection or disease but the test still outputs a positive result, this is known as a false positive. Even though this is possible, ELISA tests are definitely reliable and commonly used as well, which is considered by the immunology community.
HIV tests are one of the many examples of ELISA. It detects antibodies which are connected to the virus that are inside the blood of the patient. As for thyroid glands, Thyroid ELISA kits are used in most laboratories for further studying the present protein molecules. With this, other various kits are existent depending on what use they are.
In science and in certain industries, this test is used as one of the many tools of plant pathology and also with quality control check. Other examples this test is used for in diagnostic clinics are for food allergy and illegal drugs. The reading for this is determined by the vibrancy and intensity of the color changes in swabs whenever a sample is tested.
Two types of ELISA tests are widely used in laboratories. The first one is indirect which detects the antibodies in a given sample. An example for indirect is HIV testing, where it detects the antibodies in the sample which are against the virus. The second one is called capture or sandwich. It detects the antigens and then capture them between two antibodies. A good sample for this would be on pregnancy tests which detects the hCG or the human chorionic gonadotropin.
Blood is the usual collection, as well as urine samples. These samples are then placed in a test tube or test slide, and then sent to the laboratory for the testing and analysis. At the laboratory, it will be tested if the targeted antigen or antibody is present within the blood or urine.
The human blood samples inside the test tubes will be placed in a centrifuge to separate the different parts of it and for it to get a blood serum. A blood serum is a sample that has the clotting feature taken out of it. The high speed from the centrifuge separates the cells and plasma, making it easier to extract the serum.
Horseradish Peroxidase is an enzyme mostly used for detection where it separates Ortho Phenylenediamine Dihydrochloride and Tetramethylbenzidine. It results into a yellow color and for measuring the optical density, a plate reader would then be used. Light absorbance from OPD is 490 nanometers in wavelength, while TMB is at 450 nanometers.
If the patient has already been determined to have a disease or condition, the samples of their blood or urine will have certain antibodies which will be the ones to react to the test. These antibodies will stick to the antigens, which these antigens are bonding agents for the entire test. A solution is used to wash away everything in it except for the antigens, or also the antibodies that are sticking to the antigens.
Color changes, as mentioned before, are where you can achieve the results. Enzyme solutions are applied in the samples to get these results if they give out a positive result or not. There might be some instances where the sample from the patient has no known infection or disease but the test still outputs a positive result, this is known as a false positive. Even though this is possible, ELISA tests are definitely reliable and commonly used as well, which is considered by the immunology community.
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